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Designed specific primers for PCR assays
| Primer | Sequence (5´-3´) |
|---|---|
| Reverse transcription | |
| miR 135astem-loop | 5ˊGTCGTATGCAGAGCAGGGTCCGAGGTATTCGCACTGCATACGACTCACA 3ˊ |
| qRT-PCR | |
| miR-135a | F : 5ˊCGATATGGCTTTTTATTCCTA3ˊ |
| R : 5ˊGAGCAGGGTCCGAGGT 3ˊ | |
| Insr | F: 5ˊAACAGATGCCACTAATCCTTC 3ˊ |
| R: 5ˊGCCCTTTGAGACAATAATCC 3ˊ | |
| Vamp2 | F: 5ˊGTCACTGCCTCTGCCAAG3ˊ |
| R: 5ˊGTCCACCACCTCATCCAC3ˊ | |
qRT-PCR; Quantitive reverse transcriptase-polymerase chain reaction.
