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The effect of DMSO on eGFP expression of sheep testicular cells in A and B. 280 V/10 milliseconds, C and D. 320 V/8 milliseconds, E and F. 350 V/5 milliseconds. Testicular cells were electroporated with A., C., E. Single and B., D., F. Double bursts. Note the highest transfection efficiency 320/8 milliseconds/single burst group 48 hours after electroporation. GFP; Green fluorescent protein and DMSO; Dimethyl sulfoxide (scale bars: 90 µm).
