Table 5.
Evaluation of mean fluorescence intensity of sheep testicular cells in 350 V/5 milliseconds group after 48 hours of transfection
350 V/10 milliseconds | ||||
---|---|---|---|---|
Single burst | Double burst | |||
Testicular cells | DMSO negative | DMSO positive | DMSO negative | DMSO positive |
Myoid cells | 22.41 ± 1.9a, A | 23.69 ± 1.9a, A | 20.76 ± 2b, A | 26.28 ± 2.2c, A |
Sertoli cells | 42.63 ± 3.6a, B | 37.83 ± 3.3b, B | 43.16 ± 3.5a, B | 42.6 ± 3.7a, B |
Leydig cells | 72.72 ± 6.7a, C | 72.3 ± 6.7a, C | 76.19 ± 7b, C | 79.68 ± 7.3c, C |
SSCs | 153.35 ± 1.2a, D | 126.56 ± 1.1a, D | 134.65 ± 1.2a, D | 123.16 ± 1.2a, D |
DMSO; Dimethyl sulfoxide, SSCs; Spermatogonial stem cells, A-D; Numbers with different upper case superscript letters in the same column differ significantly, anda, b; Numbers with different lower case superscript letters in the same row differ significantly (P<0.05).