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. 2016 Aug 16;186(1):96–105. doi: 10.1111/cei.12842

Figure 2.

Figure 2

Anti‐cytomegalovirus (CMV)/pp65 T cell responses after short‐term pp65‐RNA stimulation in blood samples from anaplastic large cell lymphoma (ALCL) patients and healthy donors. CD8+ T cells (1·0 × 105) were stimulated with irradiated autologous dendritic cells (FastDCs) transfected with pp65 in‐vitro‐transcribed RNA. Microcultures were restimulated weekly for 2 weeks with the pp65‐transfected FastDCs. On day 19, pp65 responder T cells were tested in a 20‐h interferon (IFN)‐γ enzyme‐linked immunospot (ELISPOT) assay for the recognition of autologous FastDCs expressing pp65 (3–10 × 103/well). Shown are representative microcultures against autologous pp65‐transfected FastDCs from (a) four ALCL patients (NHL‐P1SU could not be tested; only patient NHL‐FVUD was CMV‐seronegative) and (b) healthy donors (only NHL‐96YC was CMV‐seronegative). Bars represent the means of duplicates ± standard deviation.