Figure 4.

Identification of human leucocyte antigen (HLA) class I‐restriction alleles of CD8⁺ T cells reactive against nucleophosmin–anaplastic lymphoma kinase (NPM–ALK) in NPM–ALK⁺ anaplastic large cell lymphoma (ALCL) patients. To assess the restricting HLA class I allele, day 19 responder T cells were tested in a parallel IFN‐γ enzyme‐linked immunospot (ELISPOT) assay against CV‐1 in origin with SV40 genes (COS‐7) cells (2·0 × 10⁴/well) co‐transfected with plasmids encoding the patients’ HLA class I alleles and NPM–ALK or cytomegalovirus (CMV)/pp65 antigen. Shown are representative microcultures reactive against COS‐7 cells co‐transfected with NPM–ALK and the individual HLA class I alleles from patients NHL‐P1SU, NHL‐4JS2 and NHL‐7AKR, after short‐term stimulation with autologous NPM–ALK transfected dendritic cells (FastDCs). Bars represent the means of duplicates ± standard deviation.