Table 1.

Primers for PCR analysis

Gene		Primer sequence (5′-3′)	Product length(bp)	Tm(°C)
dnmt1	F	5′-GCTATGTCGCCCTGGATTTC-3′	507	64.1
	R	5′-CACAGGACTCCATACCCAAGAA-3′
dnmt3a	F	5′-GGAGCACCCTTTGTTTATCG-3′	481	56.8
	R	5′-TTCGGAGGCAATGTAGCG-3′
dnmt3b	F	5′-CCACTACACCGACGTTTCCA-3′	443	60.3
	R	5′-GCCTCCACCACTTTCTCCTC-3′
mbd3	F	5′-TGGACCTCAGCACTTTCGAC-3′	387	61.6
	R	5′-GCAGAGCACTAGCGATAGCA-3′
mbd5	F	5′-GCTATGTCGCCCTGGATTTC-3′	525	63.5
	R	5′-CACAGGACTCCATACCCAAGAA-3′

Notes: F, forward primer; R, reverse primer.