Table 2.

Primers for real-time PCR

Gene		Primer sequence (5′-3′)	Product length(bp)	Tm(°C)
β-actin	F	5′-GCTATGTCGCCCTGGATTTC-3′	168	60
	R	5′-CACAGGACTCCATACCCAAGAA-3′
GADPH	F	5′-AAGGCTGAGAATGGGAAAC-3′	254	60
	R	5′-TTCAGGGACTTGTCATACTTC-3′
18S rRNA	F	5′-TTGGTGGAGCGATTTGTC-3′	129	60
	R	5′-ATCTCGGGTGGCTGAACG-3′
dnmt1	F	5′-GAAATCGACGGTCGTCTCCTC-3′	149	60
	R	5′-TCAGCAACGGCAAGCCTAAC-3′
dnmt3a	F	5′-GAGGCAATGTAGCGATCCACC-3′	160	60
	R	5′-CCAACAACCACGACCAGGAGT-3′
dnmt3b	F	5′-ACAGGCAAAGTAATCCTTGAGCG-3′	110	60
	R	5′-CCGACGTTTCCAACATCAGC-3′
mbd3	F	5′-ACCTTATTGCTGGGATGGTTTGT-3′	175	60
	R	5′-GCACGGGAAAGATGTTGATGAG-3′
mbd5	F	5′-GTTTCCATAGCCACCTCCTCC-3′	120	60
	R	5′-TGTTTCTGCCATTGACACCACT-3′

Notes: F, forward primer; R, reverse primer.