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. Author manuscript; available in PMC: 2016 Sep 5.
Published in final edited form as: Integr Biol (Camb). 2011 Jun 23;3(7):755–760. doi: 10.1039/c0ib00149j

Figure 2. Demonstration of the spatial resolution of the iFly method.

Figure 2

Trajectories are from healthy flies at day 1 after hatching and reconstructed from fly locations determined by the iFly software over a short 7.5s video segment. The fly tube has a radius of 10mm with flies traveling up to 55mm vertically. A section is magnified to provide a close-up on various intersecting fly trajectories. Velocity vectors indicate direction and amplitude of fly locomotion. Fly locations are shown as small colored dots indicating clear separation and a sub-millimeter spatial resolution. For comparison, the length of a fly body is approximately 1.5-2mm.