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. Author manuscript; available in PMC: 2016 Sep 5.
Published in final edited form as: Nat Nanotechnol. 2014 Dec 8;10(1):76–83. doi: 10.1038/nnano.2014.264

Table 1. Current blockades (ΔI) and kinetic constants (k) for single-Cys mutants.

Single-Cys αHL pores were examined with 10 μM to 100 μM SPAA at MEET concentrations from 7.5 mM to 30 mM. The buffer was 10 mM Bis-tris propane, 2 M KCl, 100 μM EDTA, pH 8.0, at 20°C. The applied potential was -50 mV. The errors are the standard deviations of the means for 'n' experiments.

PI Po→ PI PI→ Po
ΔI (pA) (k+n(104) x M-1s-1) (k-n ( 102) x M-1s-1)
(113C)1(WT)6
n= 4		 12.8 ± 0.5
13.5 ± 0.4		 0.3 ± 0.1 0.4 ± 0.1
(115C)1(WT)6
n = 3		 8.8 ± 0.2 9.0 ± 1.0 3.7 ± 0.8
(117C)1(WT)6
n = 5		 7.8 ± 0.6 2.0 ± 0.7 2.8 ± 0.2
(119C)1(WT)6
n = 3		 7.0 ± 0.4 15 ± 2 4.0 ± 1.0
(121C)1(WT)6
n = 3		 7.9 ± 0.1
7.1 ± 0.2		 5.0 ± 1.0 3.4 ± 1.7