Table 2. Current blockades (ΔI) and kinetic constants (k) for double-Cys mutants.

Double-Cys αHL pores were examined with 10 μM to 24 μM SPAA at MEET concentrations from 7.5 mM to 22.5 mM. The buffer contained 10 mM Bis-tris propane, 2 M KCl, 100 μM EDTA, pH 8.0, at 20°C. The applied potential was -50 mV. The errors are the standard deviations of the means for 'n' experiments.

	PI	PII	PO→ PI	PI → PO	PI → PII	PII →PI
	ΔI (pA)	ΔInm (pA)	(kn (104) x [M-1s-1])	(k-n (102) x [M-1s-1])	(knm[s-1] )	(knm n (102) x [M-1s-1])
(113/115)1(WT)6 n = 3	9.7 ± 1.1 10.3 ± 0.8	5.9 ± 0.7	5.0 ± 0.1 2.0 ± 0.1	0.7 ± 0.3 2.8 ± 0.2	6 ± 1 0.3 ± 0.1	0.6 ± 0.2 0.4 ± 0.3
(115/117)1(WT)6 n = 3	7.3 ± 0.4 7.8 ± 0.2	4.8 ± 0.2	20 ± 5	3.8 ± 0.7	20 ± 6	0.6 ± 0.2
(117/119)1(WT)6 n = 3	7.3 ± 0.3	4.7 ± 0.2	12 ± 1	3.0 ± 0.5	10 ± 1	0.6 ± 0.1
(119/121)1(WT)6 n = 3	6.8 ± 0.3 6.3 ± 0.3	3.9 ± 0.2	11 ± 4 6 ± 4	1.8 ± 0.6 1.5 ± 0.3	7 ± 2 120 ± 20	6 ± 1 3 ± 0.7