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. 1992 Oct 1;89(19):9306–9310. doi: 10.1073/pnas.89.19.9306

Growth and differentiation of embryonic stem cells that lack an intact c-fos gene.

S J Field 1, R S Johnson 1, R M Mortensen 1, V E Papaioannou 1, B M Spiegelman 1, M E Greenberg 1
PMCID: PMC50115  PMID: 1329091

Abstract

The c-fos protooncogene encodes a transcription factor that is thought to play a critical role in proliferation and differentiation as well as in the physiological response of mature cells to their environment. To test directly the role of c-fos in growth and differentiation, we generated mouse embryonic stem cell lines in which both copies of the c-fos gene were specifically disrupted by homologous recombination. Remarkably, the disruption of both copies of c-fos in these cells has no detectable effect on embryonic stem cell viability, growth rate, or differentiation potential. Embryonic stem cells lacking c-fos can differentiate into a wide range of cell types in tissue culture and also in chimeric mice. We conclude that despite a large body of literature suggesting an important role for c-fos in cell growth and differentiation, in at least some cell types this gene is not essential for these processes.

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Selected References

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