FIG. 2.
TSA decreases RPE cell proliferation. Cell proliferation was determined through CyQuant analysis in ARPE19 (A) and iPS-RPE (B) cells. Cells were seeded in 96-well plates at a cell density of 1 × 103 cells/well and treated with TGFβ2 (10 ng/mL) alone or in the presence of TSA (0.1 μM) for 24 h. The next day, cells were lysed according to the manufacturer's instructions to determine DNA concentrations of all treatment groups. One-way ANOVA, Bonferroni post hoc, *P < 0.05, **P < 0.01, iPS-RPE, induced pluripotent stem cell-derived RPE; RPE, retinal pigmented epithelium.