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TSA inhibits TGFβ2-mediated contraction of pRPE cells. Primary cultures of pRPE were trypsinized and seeded at a cell density of 2.5 × 105 cells/well in type I collagen (2.3 mg/mL). (A) One hour after seeding, pRPE cells embedded in collagen were treated with TGFβ2 (10 ng/mL), TSA (0.1 μM), or in combination with TGFβ2 and assessed for cell contraction 24 and 48 h. (B) Quantification of 3 independent experiments, one-way ANOVA, Bonferroni post hoc, ***P < 0.001.
