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. 2016 Sep 6;6:32628. doi: 10.1038/srep32628

Figure 2. Smad4 represses the transcription of HPSE through direct binding to its promoter.

Figure 2

(a) dual-luciferase assay showing the activity of HPSE promoter reporter and its truncates and mutant in SH-SY5Y and SK-N-SH cells. (b,c) dual-luciferase assay indicating the activity of HPSE promoter and its mutant in NB cells stably transfected with empty vector (mock), Smad4, scramble shRNA (sh-Scb), or shRNA specific for Smad4 (sh-Smad4). (d) dual-luciferase assay showing the activity of pGL3-HPSE-3.5 kb in IMR32 and SK-N-SH cells stably transfected with mock, Smad4, sh-Scb, or sh-Smad4, and those treated with BMP-2, LDN-193189, TGF-β protein, or LY364947. (e) ChIP and qPCR assay indicating the enrichment of Smad4 on HPSE promoter in IMR32 and SK-N-SH cells stably transfected with mock, Smad4, sh-Scb, or sh-Smad4, and those treated with BMP-2, LDN-193189, TGF-β protein, or LY364947. *P < 0.01 vs. pGL3-HPSE-3.5 kb, mock, sh-Scb, or IgG; ΔP < 0.01 vs. control untreated with BMP-2, LDN-193189, TGF-β protein, or LY364947.