Figure 4.

Enhanced accumulation of Ly‐6C^hi monocytes in central nervous system (CNS) of CCL2 knockout (KO) mice. (a) Frequency of Ly‐6C^hi monocytes and Ly‐6G^hi granulocytes in the brain. Frequency of Ly‐6C^hi/Ly‐6C^int monocytes and Ly‐6G^hi granulocytes in the brain were determined by flow cytometric analysis at 3 and 5 days post‐infection (dpi). (b) Accumulated number of Ly‐6C^hi/Ly‐6C^int monocytes and Ly‐6G^hi granulocytes in the brain. Accumulated number of Ly‐6C^hi/Ly‐6C^int monocytes and Ly‐6G^hi granulocytes in the brain was kinetically determined by flow cytometric analysis from 2 to 7 dpi. Left graph, Ly‐6C^hi monocytes; Middle graph, Ly‐6C^int monocytes; Right graph, Ly‐6G^hi granulocytes. (c) Accumulated number of CD3⁺ CD4⁺ and CD3⁺ CD8⁺ T cells in the brain. Values in representative dot‐plots denote average percentage of indicated cell population after gating on CD11b⁺ cells. Data in graph represent the mean ± SD of values derived from at least four mice per group. *P < 0·05; **P < 0·01; ***P < 0·001 compared between C57BL/6 and CCR2 KO or CCL2 KO mice.