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. 2016 Jul 12;149(2):186–203. doi: 10.1111/imm.12626

Figure 9.

Figure 9

CCL2 produced from haematopoietic stem cells (HSCs) plays a dominant role in central nervous system (CNS) accumulation of leucocytes. Leucocytes from the spleen, blood and brain of CCL2 knockout (KO) bone marrow (BM) chimeric models infected with Japanese encephalitis virus (JEV; 5·0 × 107 pfu) were prepared at 5 days post‐infection (dpi). (a) Frequency of Ly‐6Chi monocytes and Ly‐6Ghi granulocytes in the spleen, blood and brain. Frequency of Ly‐6Chi/Ly‐6Cint monocytes and Ly‐6Ghi granulocytes was determined by flow cytometric analysis. (b–d) Accumulated number of Ly‐6Chi/Ly‐6Cint monocytes and Ly‐6Ghi granulocytes in the spleen, blood and brain. Accumulated number of Ly‐6Chi/Ly‐6Cint monocytes and Ly‐6Ghi granulocytes was enumerated by flow cytometric analysis at 5 dpi. Left graph, Ly‐6Chi monocytes; Right graph, Ly‐6Ghi granulocytes. Values in representative dot‐plots denote average percentage of indicated cell population after gating on CD11b+ cells. Data in graph represent the mean ± SD of values derived from at least four mice per group. **P < 0·01; ***P < 0·001 compared with levels of wild‐type (WT) ‐WT BM chimeric model. # P < 0·05; ## P < 0·01 compared with levels of KO‐WT BM chimeric model.