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. 2016 Jul 12;149(2):186–203. doi: 10.1111/imm.12626

Table 1.

Specific primers for the expression of chemokines and their receptors used in real‐time quantitative RT‐PCR

Gene name Primer sequence (5′–3′)a Position cDNA GenBank ID
CCL3 FP: CCA AGT CTT CTC AGC GCC AT 158–177 NM_011337.2
RP: GAA TCT TCC GGC TGT AGG AGA AG 206–228
CCL4 FP: TTC TGT GCT CCA GGG TTC TC 128–147 NM_013652.2
RP: GAG GAG GCC TCT CCT GAA GT 388–407
CCL5 FP: CCC TCA CCA TCA TCC TCA CT 77–96 NM_013653.3
RP: CTT CTT CTC TGG GTT GGC AC 275–294
CCL7 FP: CCA GCA AGC AGC TCA ACA TT 978–998 NM_013654
RP: GCC GAT GAA GGC ATA CAA GA 1049–1069
CCL12 FP: GGG AAG CTG TGA TCT TCA GG 235–255 NM_011331.2
RP: GGG AAC TTC AGG GGG AAA TA 293–313
CCL17 FP: CAG GGA TGC CAT CGT GTT TC 223–243 NM_011332
RP: CAC CAA TCT GAT GGC CTT CTT 292–313
CXCL2 FP: ATC CAG AGC TTG AGT GTG ACG C 194–215 NM_009140.2
RP: AAG GCA AAC TTT TTG ACC GC 264–283
CXCL9 FP: TGC ACG ATG CTC CTG CA 137–154 NM_019494.1
RP: AGG TCT TTG AGG GAT TTG TAG TGG 175–199
CXCL11 FP: AAG CTC GCC TCA TAA TGC AG 323–343 NM_019494.1
RP: CAC AGT CAG ACG TTC CCA 389–407
CCR1 FP: ACC TTC GGC AGC TGT TTC A 1397–1416 NM_009912
RP: TCC ACA GAG AGG AAG GGC AG 1446–1466
CCR2 FP: TGT TAC CTC AGT TCA TCC ACG G 150–171 NM_009915.2|
RP: CAG AAT GGT AAT GTG AGC AG 446–465
CCR4 FP: TCC TGA CGG ACG TGT ACC T 325–344 NM_009916
RP: CAG ACC TAG TCC AAA AAC CCA C 412–434
CCR5 FP: TGG ATT TTC AAG GGT CAG TTC C 106–128 NM_009917
RP: GAG CCG CAA TTT GTT TCA CA 181–201
CXCR2 FP: CAT CTT ATA CAA CCG GAG CGC C 358–380 NM_009909.3|
RP: TAG TAA GGA GAT GGC TAT GCA CAC 635–659
CXCR3 FP: TGA GAC AAC TGA GGC CTC CTA 1160–1181 NM_009910
RP: TCT TGC TCC CCA GTT GAT G 1254–1274
β–actin FP: TGG AAT CCT GTG GCA TCC ATG AAA C 885–909 NM_007393.3
RP: TAA AAC GCA GCT CAG TAA CAG TCC G 1209–1233
a

FP, forward primer; RP, reverse primer.