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. 2016 Aug 16;149(2):225–237. doi: 10.1111/imm.12641

Figure 1.

Figure 1

The DNA methylation of Csf1r is decreased during macrophage maturation. Cells from murine bone marrow were matured in L929 conditioned medium and DNA was collected from cells at days 0, 3 and 5. Bisulphite sequencing, as described in the Materials and methods, was performed to assess the DNA methylation of CpG sites within the Csf1r gene, as illustrated (a). DNA methylation of CpG sites were analysed within the gene promoter (b), 1 kb upstream of fms intronic regulatory element (FIRE –1 kb) (c), and FIRE (d) regions (n = 3). *P < 0·05.