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. 2016 Aug 10;149(2):204–224. doi: 10.1111/imm.12627

Figure 4.

Figure 4

Messenger RNA expression and production of chemokines CCL2, CXCL1 and CXCL10 in the livers of murine hepatitis virus (MHV) 3‐ and MHV‐A59‐infected mice. Groups of six or seven C57BL/6 mice were intraperitoneally (i.p.) infected with 1000 TCID50 of MHV3 or MHV‐A59. At 24, 48 or 72 hr post‐infection (p.i.), livers from each group were collected. mRNA expression levels for CCL2 (a), CXCL10 (c) and CXCL1 (e) were evaluated by quantitative RT‐PCR at 24, 48 and 72 hr p.i in livers from MHV3‐ or MHV‐A59‐infected mice. Values represent fold change in gene expression relative to mock‐infected mice (arbitrary value of 1) after normalization with HPRT expression. Protein levels of CCL2 (b), CXCL10 (d) and CXCL1 (f) were quantified in livers by ELISA tests at 72 hr p.i. (*P < 0·05; **P < 0·01; ***P < 0·001).