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. 2016 Sep 6;6:32658. doi: 10.1038/srep32658

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Cytotoxicity of KCTD1 aggregates formed in the present or absence of Cu²⁺ was determined by the CCK-8 assay on HuH7 cells versus untreated control. (a) Effect of sole Cu²⁺ on HuH7 cell line proliferation. (b) KCTD1 aggregates formed in the presence of Cu²⁺ induced cell toxicity on HuH7 cells, the data shown were the mean ± s.d. of 3 independent experiments. *P < 0.05; t-test.