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. 2016 Sep 6;6:32797. doi: 10.1038/srep32797

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Optical microscopy images of freeze-dried rat brain sections brain section containing cerebellum and stem are shown in (a) control and (b) the SPC-fed group. ToF-SIMS images showing the spatial signal intensity distribution from positive ions are shown for m/z 184 (phosphocholine): (c) control and (d) the SPC-fed group; for m/z 369 (cholesterol): (e) control and (f) the SPC-fed group; and for total ions: (g) control and (h) the SPC-fed group; across an analysis area of 11 × 11 mm covering the complete tissue.