Figure 4.

Cheerio mechanosensitivity affects protein dynamics in vivo (a) Setup of the fluorescent recovery after photobleaching (FRAP) experiments showing a single ring canal where the photobleached area is marked with a white asterisk and the three areas where the fluorescence signal was followed are indicated with red lines. (b) Representative maximum intensity projections of fluorescence recovery after photobleaching of Cheerio MSR mutated flies and WT. Scale bars 1 μm. (c) FRAP recovery curves of Cheerio WT and MSR mutants. The ΔMSR and closed MSR mutants are more mobile than the WT Cheerio. On the other hand, the open MSR mutant is less dynamic than WT.