Figure 4. MiR-370-3p binds to the 3′-UTR of MGMT mRNA and influences DNA pathway protein expression.

(A) A luciferase report system vector was constructed, which included a wild-type 3′-UTR fragment of MGMT or mutant miR-370-3p binding sequence. (B) Luciferase activity was detected in LN18 cells co-transfected with a luciferase reporter system and miR-370-3p or miR-NC. (C) Following TMZ treatment, DNA pathway proteins in different treatment of GBM cells were evaluated with the Western blot analysis. β-actin was used as an internal control. (D) Correlation of miR-370-3p and MGMT expression in patients. Each experiment was performed in triplicate with *P < 0.05, compared to negative controls.