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. 2016 Sep 6;6:32770. doi: 10.1038/srep32770

Figure 3. Inhibition of Wnt/β-catenin signalling suppresses KIF3A-knockdown phenotypes.

Figure 3

(a) ICG-001-mediated suppression of Wnt/β-catenin hyper activation in KIF3A-depleted cells. Cells were transfected with TOPFlash or FOPFlash, and treated with DMSO or ICG-001 (7 μM) for 24 h. Luciferase reporter activity was calculated by dividing TOP/RL ratio by FOP/RL ratio (n = 5, error bars indicates SEM; p-value was determined by unpaired t-test). (b) Suppression of the active proliferation of KIF3A-depleted cells by ICG-001. Cell proliferation was measured by CCK assay for 4 days (n = 8; error bars indicate SEM; p-value was determined by one-way ANOVA). (c) Quantitative RT-PCR analysis of stemness markers, Nanog, OCT4 and SOX2 after ICG-001 treatment (n = 3; error bars indicate SEM; p-value was determined by two-way ANOVA). GAPDH was used as an internal control. *P < 0.05; **P < 0.01.