Figure 1. Experimental flow and verification of the OVA-induced mucus overproduction model.

(A) Schematic flow chart of the experimental design. (B) Validation test of serum IgE assay using blood samples collected on day 24 in the sham (n = 40) and OVA groups (n = 112). The bars represent means ± S.E.M. Significance differences were determined by two-tailed, unpaired Student’s t-test, ***indicates P < 0.01. (C) Morphological changes of the lung from sham and OVA-treated mice. The sections were stained with hematoxylin and eosin (H&E) and analyzed with a microscope at a magnification of ×63. ASM, airway smooth muscle cells hypertrophy and hyperplasia; AW, airway; GCH, goblet cell hyperplasia; M, excess mucus secretion.