Fig. 1.

Increased expression of WT APOL1 inversely correlates to HIV-1 persistence in CIHPs. a Summary graph of statistical dot plots (left panel) showing the co-localization off WT APOL1 with Rab5, Rab7, EEA1 and LAMP1 as an average measured by Pearson’s Coefficient on data analyzed with the Olympus FLUOVIEW FV1000 confocal microscope software (N = 3). Representative fluorescent microscopic images (right panel) showing the co-localization of WT APOL1 (red) with Rab5, Rab7, EEA1 and LAMP1 (green) marked vesicles and plasma membrane in CIHPs. Nucleuses are stained with DAPI in blue. b Statistical histogram graph showing the dose-dependent response of APOL1 gene expression after IFN-γ stimulation (16 h). Results are expressed as relative fold change in IFN-γ treated CIHPs versus untreated controls (Ctrl) and normalized to GAPDH gene expression (N = 4). c Statistical histogram graph showing HIV-1 accumulation in CIHPs incubated for 16 h with IFN-γ (10 ng/mL) compared to their untreated counterparts (Ctrl). Results are expressed as relative fold change of HIV-1 DNA RU5 in IFN-γ treated cells versus Ctrl and normalized to GAPDH gene (N = 4). P values *<0.05, **<0.01; ***<0.001