Table 3.
Reactive oxygen species (ROS) production by bovine spermatozoa [RLU/s/106 sperm] affected by four doses of lycopene (LYC), untreated vs. treated with ferrous ascorbate (FeAA)
| Fractions | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Fractions untreated with FeAA | Fractions treated with FeAA | ||||||||
| Ctrl 1 (SC Ctrl) | 2 mmol/L LYC | 1 mmol/L LYC | 0.5 mmol/L LYC | 0.25 mmol/L LYC | Ctrl 2 (FeAA Ctrl) | 2 mmol/L LYC | 1 mmol/L LYC | 0.5 mmol/L LYC | 0.25 mmol/L LYC |
| Time 0 h | |||||||||
| 1.63 ± 0.32 | 1.01 ± 0.22 | 1.21 ± 0.30 | 1.30 ± 0.16 | 1.57 ± 0.30 | 4.22 ± 0.70**1 | 2.00 ± 0.49*2 | 2.12 ± 0.41*2 | 2.55 ± 0.39 | 3.16 ± 0.58*1 |
| Time 2 h | |||||||||
| 4.49 ± 0.42 | 2.49 ± 0.72 | 2.58 ± 0.50 | 3.66 ± 0.19 | 4.10 ± 0.25 | 9.75 ± 0.85***1 | 5.28 ± 0.22**2 | 5.79 ± 0.45**2 | 7.90 ± 0.48*1 | 8.11 ± 0.65**1 |
| Time 6 h | |||||||||
| 10.92 ± 0.62 | 6.55 ± 0.21**1 | 7.01 ± 0.54**1 | 8.66 ± 0.52 | 9.15 ± 0.36 | 23.55 ± 1.51***1 | 12.33 ± 1.09***2 | 14.77 ± 0.80***2 | 17.00 ± 1.21*2, **1 | 20.25 ± 1.56***1 |
Mean ± Standard Error
*P < 0.05; **P < 0.01; ***P < 0.001
1 – vs. Control 1 (SC Control)
2 – vs. Control 2 (FeAA Control)