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. 2016 Sep 6;13(1):64. doi: 10.1186/s12977-016-0298-1

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Mutation of S40 to Ala minimally affects virus budding, maturation, or infectivity. a–c Electron microscopy of particles associated with cells transfected with pEnv and pNL4-3ΔEnv-WT (a), -S40A (b), or -S40F (c). Particle exhibiting mature morphology were quantified compared to WT (f). d p24 capsid antigen detected in culture media, as indicated by triplicate ELISA; e Viral particle infectivity, determined by MAGI assay, done in triplicate