Fig. 4.

Impact of Gagp6 S40A/Pol p6*-PR F56C mutation on IDV susceptibility. 293T cells were co-transfected with DNA encoding the indicated construct and a plasmid encoding VSV G protein as described in “Methods”. IDV was present at 0 (a) or 0.01 μM (b). Viral supernatants were collected at 48 h post-transfection and serial dilutions were used in triplicate to infect 1 × 10⁴ TZM-bl cells in 96-well plates. Infectivity of the virus particles in TZM-bl cells was assessed at 48 h post-infection using the reporter β-galactosidase