Fig. 6.

Model precursor autoprocessing in the presence of IDV. a Western blot analysis of the WT (left) and F56C (right) fusion precursors. Post-nuclear total lysates were resolved by SDS-PAGE and analyzed by Western blotting. Each blot was divided into two parts with 25 kD as the cutoff size and then probed separately with mouse Flag and GAPDH (top) or HA (bottom) antibodies. Both blots were probed with IR800 goat anti-mouse secondary antibody and scanned using a LI-COR Odyssey scanner. The brackets on the right indicate the autoprocessing products resulted from the proximal (P) and distal (D) site cleavages. b–f Quantification of autoprocessing products. The images shown are representative of nine experiments