Fig. 7.
Trans cleavage at the proximal site in the presence of IDV. a Schematic drawing of a model precursor substrate and expected products of trans processing by enzymatically active miniprecursors. b, c Quantification of trans processing product and p6*-PRb enzyme. Band intensity was used to reflect trans processing efficiency and enzyme detection levels. d–g Western blot analyses of the model substrate and four fusion precursors with increasing IDV. Post-nuclear total lysates were resolved on SDS-PAGE and transferred to a PVDF membrane. Each blot was simultaneously probed with mouse HA (top) or rabbit V5 (bottom) antibodies. The blots were then probed with IR800 goat anti-mouse secondary and IR700 goat anti-rabbit antibody and scanned using a LI-COR Odyssey scanner. The images shown are representative of four experiments