Fig. 4.

T lymphocyte intracellular cytokine staining in human MM and Vk*MYC mouse models. a Human PBMC CD4⁺/CD8⁺ T lymphocyte intracellular cytokine staining (ICS). Box and whisker plots showing (i) % IFNγ⁺ (top) and % IL-17A⁺ (bottom) expressing CD4⁺ T lymphocytes (ii) % IFNγ⁺ expressing CD8⁺ T cells. Clear boxes ARCBS donors, light grey boxes NDMM, dark grey boxes RRMM. b ICS matched pair analysis in PBMC and BMMC samples in NDMM (as described for a). (i) % IFNγ⁺ (top) and % IL-17A⁺ (bottom) expressing CD4⁺ T lymphocytes (ii) % IFNγ⁺ expressing CD8⁺ T cells. c Murine BMMC CD4⁺/CD8⁺ T lymphocyte ICS in Vk*MYC mouse models. Box and whisker plots showing (i) % IFNγ⁺ (top) and % IL17⁺ T cells (bottom) expressing CD4⁺ T lymphocytes (ii) % IFNγ⁺ expressing CD8⁺ T cells. (Left) Transgenic Vk*MYC, (Right) Transplant Vk#31. Closed circles represent a-m WT, open circles represent diseased mice (which are subdivided into low, intermediate (int.) or high-grade disease as described in Fig. 2a). d Box and whisker plots showing IFNγ (top) and IL17 (bottom) concentration in BM supernatants from transplant Vk#31 and age-matched WT controls by cytometric bead array (CBA) analysis