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Analysis of the five TcTre transcripts in different tissues of last-instar larvae using semi-quantitative PCR (RT-PCR). Total RNA was extracted from various tissues: fat body (Fb), midgut (Mg), cuticle (Cu) and Malpighian tubules (Mt). The expression of Tcβ-actin in the four tissues was used to standardize the relative expression levels of the five TcTre genes. CK represents PCR products without a template used as the controls
