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. 2016 Sep 6;16(1):67. doi: 10.1186/s12896-016-0297-2

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Effects on key genes in the chitin biosynthesis pathway after specific RNAi in last-instar larvae at 48 and 72 h. The levels of these genes relative to the Tcβ-actin mRNA level were measured via qRT-PCR. a mRNA levels of glycogen synthase (GS) and glycogen phosphorylase (GP). b mRNA levels of two hexokinases (HK1 and HK2). c mRNA levels of three chitin synthases (CHS1a, CHS1b and CHS2)