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. 2016 Sep 5;17(1):708. doi: 10.1186/s12864-016-3030-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — The artificial mixture, variant calling, and phasing of variants for an influenza A(H3N2) M gene. Donor viruses (1) are mixed (2) in a 99:1 ratio with new variants called (3) for the mixed virus and pairwise tested for phasing and visualization (4) by heat map. Consensus and minority phases are colored red and blue corresponding to the consensus alleles of each parent donor virus. Single nucleotide variants are shown with a triangle and colored according to their phase. Independent phase SNVs—without linkage to other minority variants—have green and gray triangles