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. 2016 Sep 6;6:28281. doi: 10.1038/srep28281

Figure 4. The effect of miR-21-5p on the EMT phenotype of keratinocytes from keloid and normal skin.

Figure 4

After keloid keratinocytes and normal skin keratinocytes were transfected with miR-21-5p mimic or inhibitor and their corresponding negative controls for 72 or 48 hours, respectively, the expressions of EMT-related markers E-cadherin, Vimentin, Snail1 and Snail2 in keloid keratinocytes and normal skin keratinocytes were examined by qRT-PCR (a,b,d,e) and western blot analysis (c,f ), and were normalized by the expression of GAPDH. The western blot data are expressed as fold change relative to the mean value of control group. Statistical significance was obtained using Independent-Samples t-test for qRT-PCR and Paired t-test for western blot analysis. Data was shown as the mean ± SEM of 3 independent cells from 3 replicates. (*p < 0.05, **p < 0.01).