Fig. 1.

Mitochondrial (mt) genome organization in Globodera ellingtonae. a PCR amplification products of mtDNA used in cloning and sequencing: amplicons of 1) ‘outward’ amplicon of mtDNA-I, 2) ‘inward’ amplicon of mtDNA-I, 3) ‘outward’ amplicon of mtDNA-II, 4) ‘inward’ amplicon of mtDNA-II, and 5) 1 kb plus DNA ladder (Invitrogen). b Southern blots confirm the size and organization of the two circles. Symbols above each lane correspond to those in panel c and represent the restriction enzymes used to digest total DNA: a star for EcoRI, a triangle for KpnI, and a circle for PacI. Blots hybridized with (i) probe to shared sequence, exposure 1 min; (ii) probe to mtDNA-I specific sequence, exposure 4 min; (iii) probe to mtDNA-II specific sequence, exposure 30 s; and (iv) image of 1 kb plus ladder from ethidium bromide stained gel aligned to x-ray images. c Organization of G. ellingtonae mitochondrial genome. Protein-coding, rRNA, and pseudo- gene abbreviations are located next to arced arrows showing their location and direction. Single letter abbreviations of amino acids denote locations of associated tRNA genes. Locations of PCR products used in cloning and sequencing the circles and of probes used in Southern hybridizations are shown. Locations of nucleotides 1 and 1000 are noted on each circle for size reference. The star, triangle, and square indicate restriction enzyme sites as denoted above