Table 1.
Transformation of P. palmivora using various concentrations of A. tumefaciens EHA105 expressing pCB301TOR-GFP
| OD600 of Agrobacteria | Data from 1 representative experiment | Data from 3 experiments | ||||
|---|---|---|---|---|---|---|
| Zoospore concentration (/ml) | Zoospore volume (ml) | Number of G418-resistant transformants | Number of transformants with detectable GFP signal | Number of G418-resistant transformants/107 zoospores | Average number of G418-resistant transformants/107 zoospores | |
| 0 | 3.3 × 106 | 2.5 | 0 | 0 | 0 | 0 |
| 0.1 | 3.3 × 106 | 2.5 | 13 | 10 | 16 | 22 ± 5 |
| 0.2 | 3.3 × 106 | 2.5 | 16 | 15 | 19 | 21 ± 2 |
| 0.4 | 3.3 × 106 | 2.5 | 25 | 23 | 30 | 27 ± 5 |
| 0.8 | 3.3 × 106 | 2.5 | 13 | 13 | 16 | 18 ± 4 |