Table 4.
Comparison of ATP requirements for C. thermocellum and model organisms during anaerobic growth
| ATP requirement (mmol ATP/g DCW) | |||
|---|---|---|---|
| C. thermocellum | E. coli [46] | S. cerevisiae [99] | |
| Polysaccharides/cell wall | 7.16 | 2.05 | 4.63 |
| Protein | |||
| Amino acid synthesis | 5.65 | 1.36 | 1.81 |
| Polymerization | 17.49 | 19.14 | 17.76 |
| Lipid | 0.52 | 0.14 | 0 |
| RNA | |||
| Nucleoside monophosphate formation | 1.20 | 3.45 | 1.35 |
| Polymerization | 0.27 | 0.92 | |
| mRNA turnover | 0.30 | 1.39 | 0.71 |
| DNA | |||
| Deoxynucleoside monophosphate formation | 0.48 | 0.86 | |
| Polymerization | 0.18 | 0.19 | |
| Subtotal | 33.23 | 29.50 | 26.26 |
| Transport | |||
| Ammonium | 8.43 | 4.24 | 6.29 |
| Potassium | 0.20 | 0.19 | 2.4 |
| Phosphate | 0.80 | 0.77 | |
| Total ATP required | 42.66 | 34.71 | 34.95 |
| Y MAXATP (g DCW/mol ATP) | 23.44 | 28.81 | 28.61 |