Fig. 2.

p53 interference reduces the CPS-induced cell death in mutp53-expressing cancer cells. a U373 and SKBR3 were transfected with pSuper (si-ctr) or pSuper-p53 (si-p53) and 36 h after transfection equal amount of total cell extracts was analysed by western immunoblotting with anti-p53 antibody. Anti-β-actin was used as protein loading control. The percentage of p53 reduction was measured by densitometry and plotted in the right panel. b Control and interfered cells as in (a) were treated with CPS (200 μM). Twenty-four hours later, the percentage of dead cells was scored by trypan blue staining. Data are the mean ± S.D. of three independent experiments performed in triplicate. *P = 0.001