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. 2016 Sep 6;11(1):61. doi: 10.1186/s40793-016-0185-3

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Maximum likelihood phylogenetic tree inferred from three housekeeping genes. The phylogenetic relationships inferred from the alignment of 8077 bp of concatenated DNA from three housekeeping genes highlighting the positions of S. plymuthica 3Rp8 and 3Re4-18 relative to their closest Serratia strains for which complete genomes are publicly available. A representative rhizosphere bacterium from the genera Pseudomonas was used as outgroup. For the construction of the tree, the protein-coding house-keeping genes gyrB (2420 bp), rpoP (4146 bp) and nusA (1511 bp) were concatenated and aligned. Then the evolutionary history was inferred by using the Maximum Likelihood method based on the Tamura-Nei model [35]. The percentage of trees in which the associated taxa clustered in the bootstrap test (1000 replicates) is shown next to the branches [36]. Evolutionary analyses were conducted in MEGA7 [37]