Figure 1. Derivation of U2AF35(S34F)-transformed Cell Lines.
(A) Proliferation of parental Ba/F3 cells cultured in the presence (+) or absence (−) of IL-3, and Ba/F3-V, Ba/F3-U2AF35, Ba/F3-U2AF35(S34F) or Ba/F3-BCR-ABL cells cultured in the absence of IL-3. A representative graph from n=9 biological replicates is shown. Two other replicates are shown in Figure S1B.
(B) Proliferation of parental Ba/F3 cells, and established Ba/F3-V, Ba/F3-U2AF35, Ba/F3-U2AF35(S34F) or Ba/F3-BCR-ABL cells.
(C) Tumor formation in mice (n=4 mice per group) injected with Ba/F3-V or Ba/F3-U2AF35(S34F) cells. Error bars indicate SD. *P<0.05; **P<0.01. See also Figure S1.