Fig. 4.

rHIgM22 improves number of retrograde-labeled brainstem neurons. FluoroGold-labeled neurons were counted in brainstem sections that correspond to the (2.5 × 2.5 × 2.5) mm³ voxel used for collecting MRS data. a Example of a cluster of fluorescently labeled neurons in the brainstem is shown. Extensive labeling of cell bodies as well as axons and dendrites can be easily appreciated. b The number of labeled neurons in the uninfected mice (N = 3) is shown as a reference. rHIgM22 treatment increased the number of retrograde-labeled brainstem neurons compared to the saline-treated group (p = 0.011, t test). The average number of retrograde-labeled brainstem neurons ± SEM per treatment group is shown. Symbols represent the treatment groups: rHIgM22 (red triangles) and saline (green boxes). c Number of fluorescently labeled neurons correlated positively and significantly to the number of axons (p = 0.008, R ² = 0.24)