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. Author manuscript; available in PMC: 2016 Sep 6.
Published in final edited form as: Biotechniques. 2011 Apr;50(4):229–234. doi: 10.2144/000113652

Figure 4. Quantitative analysis of macrophage burden in EAE spinal cord tissue using real-time PCR in a single rat.

Figure 4

(A) Correlation between IFI, obtained from 19F NMR, and CD68 mRNA levels. Data shows a linear correlation (R = 0.89), consistent with the PFC internalization into macrophage. The mRNA levels are normalized to gapdh. (B) Plot of IFI versus GFAP mRNA shows no correlation (R = −0.13), suggesting that PFC is not taken up by astrocytes.