Table 1. Macromolecule-production information.
Source organism | S. frigidimarina NCIMB 400 |
DNA source | S. frigidimarina NCIMB 400 |
Forward primer (5′–3′) | ATG AAT AAC CAA TCA GCT AAA AAA TCT CC |
Reverse primer (5′–3′) | CTA CAA CGG CTG CAT CTG CTT TTG |
Cloning vector | pETBlue-1 (Novagen) |
Expression vector | pETBlue-1 (Novagen) |
Expression host | E. coli Tuner (DE3) pLacI |
Complete amino-acid sequence of the construct produced | MNNQSAKKSPTRLTYISDIIEISPYLRRLVLSGEQLANFPADQQGAYVKVLIPQPGETTVNMTLTGPNAAIKRSYTIREFDPVRGQLSLDFVINKHTGPATDWAKLANVGDTVAIAGPGPLKMNRFDFNDYLLFGDSTSINAVDALIKRLPATAKGHIIMLVNSHQEQALLSQHPLLKTHWLVLNDSITAEQQIDWLLDKLELFGDLPAVTQVFVGLEATQVRVIKQYLLEQQQLPLSSISATGYWKRNTDADTFGKQKQMQPL |
Theoretical pI† | 7.6 |
Calculated using Protein Calculator (http://protcalc.sourceforge.net/).