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. 2016 Aug 9;72(Pt 9):667–671. doi: 10.1107/S2053230X16011419

Table 1. Macromolecule-production information.

Source organism S. frigidimarina NCIMB 400
DNA source S. frigidimarina NCIMB 400
Forward primer (5′–3′) ATG AAT AAC CAA TCA GCT AAA AAA TCT CC
Reverse primer (5′–3′) CTA CAA CGG CTG CAT CTG CTT TTG
Cloning vector pETBlue-1 (Novagen)
Expression vector pETBlue-1 (Novagen)
Expression host E. coli Tuner (DE3) pLacI
Complete amino-acid sequence of the construct produced MNNQSAKKSPTRLTYISDIIEISPYLRRLVLSGEQLANFPADQQGAYVKVLIPQPGETTVNMTLTGPNAAIKRSYTIREFDPVRGQLSLDFVINKHTGPATDWAKLANVGDTVAIAGPGPLKMNRFDFNDYLLFGDSTSINAVDALIKRLPATAKGHIIMLVNSHQEQALLSQHPLLKTHWLVLNDSITAEQQIDWLLDKLELFGDLPAVTQVFVGLEATQVRVIKQYLLEQQQLPLSSISATGYWKRNTDADTFGKQKQMQPL
Theoretical pI 7.6

Calculated using Protein Calculator (http://protcalc.sourceforge.net/).