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. 2016 Aug 9;72(Pt 9):667–671. doi: 10.1107/S2053230X16011419

Table 2. Crystallization conditions.

Method Hanging drop
Plate type EasyXtal 15-Well Tool (Qiagen)
Temperature (°C) 18
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 20 mM Tris–HCl pH 7.6, 50 mM NaCl
Composition of reservoir solution 22% PEG 3350, 0.22 M magnesium formate dihydrate
Volume and ratio of drop 2 µl, 1:1
Volume of reservoir (µl) 500