Table 2. Crystallization conditions.
Method | Hanging drop |
Plate type | EasyXtal 15-Well Tool (Qiagen) |
Temperature (°C) | 18 |
Protein concentration (mg ml−1) | 10 |
Buffer composition of protein solution | 20 mM Tris–HCl pH 7.6, 50 mM NaCl |
Composition of reservoir solution | 22% PEG 3350, 0.22 M magnesium formate dihydrate |
Volume and ratio of drop | 2 µl, 1:1 |
Volume of reservoir (µl) | 500 |