Table 5. Properties of known bacterial PDCs in order of decreasing thermostability.

	ZpPDC	ApPDC	ZmPDC	GdPDC	GoPDC	SvPDC
Gram status	Negative	Negative	Negative	Negative	Negative	Positive
Amino-acid identity (%)	Reference	73	63	71	67	31
Temperature optimum (°C)	65	65†	60†	45–50‡	53§	NA
Temperature dependence of activity retention	60°C, 100%	50°C, 100%	45°C, 85%	NA (half-life at 60°C, 0.3 h‡)	55°C, 98%	45°C, 95%
	65°C, 80%†	60°C, 65%	60°C, 65%		60°C, 70%	50°C, 0%¶
	70°C, 0%	65°C, 45%	65°C, 45%		65°C, 40%§
		70°C, 5%¶	70°C, 0%¶
Kinetics	Michaelis–Menten	Michaelis–Menten	Michaelis–Menten	Michaelis–Menten	Michaelis–Menten	Sigmoidal
V max (U mg−1)	165 ± 3 (pH 6.5)	110 ± 2 (pH 6.5)†	121 (pH 6.5)†	20 (pH 5)	57 (pH 5)	103
	116 ± 2 (pH 6.5)†	97 (pH 5)¶	100 (pH 6)¶	39 (pH 6)	47 (pH 6)	45 (pH 6.5)¶
	130 (pH 6)¶	79 (pH 7)¶	78 (pH 7)¶	43 (pH 7)‡	125 (pH 7)§	35 (pH 7)¶
	140 (pH 7)¶		120
			181†
K m (S0.5) (mM)	0.67 ± 0.05 (pH 6.5)	2.8 ± 0.2 (pH 6.5)†	1.3 (pH 6.5)†	0.06 (pH 5)	0.12 (pH 5)	13
	2.5 ± 0.2 (pH 6.5)†	0.39 (pH 5)¶	0.43 (pH 6)¶	0.6 (pH 6)	1.2 (pH 6)	5.7 (pH 6.5)
	0.24 (pH 6)¶	5.1 (pH 7)¶	0.94 (pH 7)¶	1.2 (pH 7) ‡	2.8 (pH 7)§	4.0 (pH 7)¶
	0.71 (pH 7)¶		0.31 (pH 6)‡
			1.1
			0.4 (pH 6)†
PDB entry	5euj	2vbi	1zpd	4cok	NA	NA
GenBank gene	AF474145	AF368435.1	M15393.2	KJ746104.1	KF650839.1	AAL18557.1
GenBank protein	AAM49566.1	AAM21208.1	AAA27696.2	AIG13066.1	AHB37781.1	AF354297.1
R.m.s.d.	Reference	0.70	0.70	0.62	NA	NA
Q scores	Reference	0.94	0.90	0.94	NA	NA

^†Gocke et al. (2009 ▸).

^‡van Zyl, Schubert et al. (2014 ▸).

^§van Zyl, Taylor et al. (2014 ▸).

^¶Raj et al. (2002 ▸).

^††Lowe & Zeikus (1992 ▸).

^‡‡Siegert et al. (2005 ▸).

^§§Bringer-Meyer et al. (1986 ▸).

^¶¶Meyer et al. (2010 ▸).