Table 1. Macromolecule-production information.

F indicates forward and R reverse for the primers.

Source organism	Human	Human
Construct	Final 329–440 (first 329–671, then 6×His-SUMO-329–440)	Final 316–440 (first 316–671, then 6×His-SUMO-316–440)
DNA source	pET-15b transposase domain of SETMAR (wild type)	pFLAG-CMV4 full-length SETMAR (wild type)
329-671_F (left) and 316-671_F (right) primers	ATATCGGATCCATGAAAATGATGTTAGACAAAAAGCAAATTCG	TATATCGGATCCGTGTTCCCCTCCTGCAAGCGATTGA
329-671_R (left) and 316-671_R (right) primers	TATAGCTCGAGTTAATCAAAATAGGAACCATTACAATC	TATAGCTCGAGTTAATCAAAATAGGAACCATTACAATC
DBD_F primer	GATATACCATGGGCAGCAGCCATCATCATCATCATC	GATATACCATGGGCAGCAGCCATCATCATCATCATC
DBD_R primer	TATAGCTCGAGTTACACCTTTCCAATTTGCTTCAAATGTC	TATAGCTCGAGTTACACCTTTCCAATTTGCTTCAAATGTC
Cloning vector	p6×His-SUMO	p6×His-SUMO
Expression vector	p6×His-SUMO	p6×His-SUMO
Expression host	E. coli Rosetta (DE3)	E. coli Rosetta (DE3)
Complete amino-acid sequence	SMKMMLDKKQIRAIFLFEFKMGRKAAETTRNINNAFGPGTANERTVQWWFKKFRKGDESLEDEERSGRPSEVDNDQLRAIIEADPLTTTREVAEELNVNHSTVVRHLKQIGKV	SVFPSCKRLTLETMKMMLDKKQIRAIFLFEFKMGRKAAETTRNINNAFGPGTANERTVQWWFKKFSKGDESLEDEERSGRPSEVDNDQLRAIIEADPLTTTREVAEELNVNHSTVVRHLKQIGKV