Table 2. Crystallization.
ND, not determined.
| Protein–DNA complex | DBD (329–440)–TIR2 | DBD (329–440) (C381S)–TIR2 | DBD (329–440) (C381S)–variant TIR1 | DBD (316–440) (C381S)–variant TIR1 | DBD (329–440) (C381R)–TIR2 |
|---|---|---|---|---|---|
| Method | Vapor diffusion | Vapor diffusion | Vapor diffusion | Vapor diffusion | Vapor diffusion |
| Image | Figs. 2 ▸(a) and 2 ▸(b) | Fig. 2 ▸(c) | Fig. 2 ▸(d) | Fig. 2 ▸(e) | Fig. 2 ▸(f) |
| Plate type | VDX | VDX | VDX | VDX | VDX |
| Temperature (K) | 293 | 293 | 293 | 293 | 293 |
| Protein concentration | 0.5 mM:0.6 mM | 0.5 mM:0.6 mM | 0.5 mM:0.6 mM | 0.5 mM:0.6 mM | 0.5 mM:0.6 mM |
| Buffer composition of protein solution | 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT | 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT | 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT | 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT | 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT |
| Composition of reservoir solution | 0.2 M Li2SO4, 0.1 M bis-tris pH 5.5, 25%(w/v) PEG 3350 | 25% poly(acrylic acid sodium) 5100, 0.10 M HEPES pH 7.5, 0.02 M MgCl2 | 0.01 M Mg(CH3COO)2, 0.05 M MES pH 5.6, 2.5 M (NH4)2SO4 | 0.10 M Mg(HCO2)2, 15% PEG 3350 | 0.025 M MgSO4 hydrate, 0.05 M Tris–HCl pH 8.5, 1.8 M (NH4)2SO4 |
| Volume and ratio of drop | 2 µl, 1:1 | 2 µl, 1:1 | 2 µl, 1:1 | 2 µl, 1:1 | 2 µl, 1:1 |
| Volume of reservoir (ml) | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| Space group | ND | C2 | ND | C2221 | C2221 |