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. 2016 Aug 26;72(Pt 9):720–725. doi: 10.1107/S2053230X16013017

Table 2. Crystallization.

Method Vapour diffusion
Plate type Hanging drop
Temperature (K) 293
Protein concentration (mg ml−1) 20
Buffer composition of protein solution 25 mM Tris–HCl pH 8.0, 100 mM NaCl
Composition of reservoir solution 0.1 M bis-tris pH 5.5, 20% PEG 3350
Volume and ratio of drop 2 µl, 1:1
Volume of reservoir (ml) 1